X-Ray NMR Methods

Dvir H, Choe S. (2009) Bacterial expression of a eukaryotic membrane protein in fusion to various Mistic orthologs. Protein Expr Purif., 68, 28-33.

Dvir H, Lundberg ME, Maji SK, Riek R, Choe S. (2009) Mistic: cellular localization, solution behavior, polymerization, and fibril formation. Protein Sci., 18, 1564-70.

Maslennikov I, Krupa M, Dickson C, Esquivies L, Blain K, Kefala G, Choe S, Kwiatkowski W. (2009) Characterization of protein detergent complexes by NMR, light scattering, and analytical ultracentrifugation. J. Struct. Funct. Genomics., 10, 25-35.

Kefala, G., Kwiatkowski, W., Esquivies, L., Maslennikov, I., Choe, S. (2007) Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli. J. Struct. Funct. Genomics., 8, 167-72.

Maslennikov, I., Kefala, G., Johnson, C., Riek, R., Choe, S., Kwiatkowski, W. (2007) NMR spectroscopic and analytical ultracentrifuge analysis of membrane protein detergent complexes. BMC Struct Biol. 7:74.

Tzakos AG, Grace CR, Lukavsky PJ, Riek R. (2006) NMR techniques for very large proteins and rnas in solution. Annu Rev Biophys Biomol Struct. 35, 319-42.

Pegan, S., Kwiatkowski, W., Choe, S., Riek, R. (2003) High-throughput backbone resonance assignment of small 13C,15N-labeled proteins by a triple-resonance experiment with four sequential connectivity pathways using chemical shift-dependent, apparent 1J(1H,13C): HNCACB(coded)HAHB. J.Magn.Reson., 165, 315-319.

Kwiatkowski, W. and Riek, R. (2003) Chemical shift-dependent apparent scalar couplings: An alternative concept of chemical shift monitoring in multi-dimensional NMR experiments. J. Biomol. NMR 25, 281-290.

Riek, R., Pervushin, K., Fernandez, C., Kainosho, M. and Wüthrich, K. (2001). [13C,13C]- and [13C,1H]-TROSY in a triple resonance experiment for ribose-base and intrabase correlations in nucleic acids. J.Am.Chem.Soc. 123, 658-664.

Riek, R. (2001) Enhancement of the steady-state magnetization in TROSY experiments. J. Biomol. NMR 21, 99-105.

Pervushin, K., Fernandez, C., Riek, R., Ono, A., Kainosho, M. and Wüthrich, K. (2000). Determination of (h2)J(NN) and (h1)J(HN) coupling constants across Watson-Crick base pairs in the Antennapedia homeodomain-DNA complex using TROSY. J. Biomol. NMR 16, 39-46.

Kwiatkowski, W., Noel, J.P., and Choe, S. (2000) Use of Cr Ka radiation to enhance the signal from anomalous scatterers including sulfur. J.Appl.Cryst. 33, 876-881.

Riek, R., Pervushin, K. and Wüthrich, K. (2000). TROSY and CRINEPT:NMR with large molecular and supramolecular structures in solution. TiBS 25, 462-468.