TGF-Beta and GPCR Signaling

Keshwani MM, Klammt C, von Daake S, Ma Y, Kornev AP, Choe S, Insel PA, Taylor SS. (2012) Cotranslational cis-phosphorylation of the COOH-terminal tail is a key priming step in the maturation of cAMP-dependent protein kinase. Proc Natl Acad Sci U S A. 109(20), E1221-9. Epub 2012 Apr 9.

Allendorph, G.P., Read, J.D., Kawakami, Y., Kelber, J.A., Isaacs, M.J., Choe, S. (2011) Designer TGF-beta superfamily ligands with diversified functionality PLoS One. 6, e26402.

Klammt, C., Perrin, M.H., Maslennikov, I., Renault, L., Krupa, M., Kwiatkowski, W., Stahlberg, H., Vale, W., Choe, S. (2011) Polymer-based cell-free expression of ligand-binding family B G-protein coupled receptors without detergents. Protein Sci. 20, 1030-41.

Blain, K.Y., Kwiatkowski, W., Choe, S. (2010) The functionally active Mistic-fused histidine kinase receptor, EnvZ. Biochemistry 49,9089-95.

Valera, E., Isaacs, M.J., Kawakami, Y., Izpisua Belmonte, J.C., Choe, S. (2010) BMP-2/6 heterodimer is more effective than BMP-2 or BMP-6 homodimers as inductor of differentiation of human embryonic stem cells. PLoS One, 5, e11167.

Maslennikov, I., Klammt, C., Hwang, E., Kefala, G., Okamura, M., Esquivies, L., Mors, K., Glaubitz, C., Kwiatkowski, W., Jeon Y.H., Choe, S. (2010) Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis. Proc. Natl. Acad. Sci. USA, 107, 10902-7.

Isaacs, M.J., Kawakami, Y., Allendorph, G.P., Yoon, B.H., Izpisua Belmonte, J.C., Choe, S. (2010) Bone Morphogenetic Protein-2 and -6 Heterodimer Illustrates the Nature of Ligand-Receptor Assembly. Mol. Endocrinol. 24, 1469-77.

Xie, W., Blain, K.Y., Kuo, M.M., Choe, S. (2010) Protein Engineering of Bacterial Histidine Kinase Receptor Systems. Protein Pept. Lett. 17, 867-73.

Yang, F., West, A.P. Jr., Allendorph. G.P., Choe, S., Bjorkman, P.J. (2008) Neogenin Interacts with Hemojuvelin through Its Two Membrane-Proximal Fibronectin Type III Domains. Biochemistry, 47, 4237-45.

Perrin, M.H., Grace, C.R., Digruccio, M.R., Fischer, W.H., Maji, S.K., Cantle, J.P., Smith, S., Manning, G., Vale, W.W., Riek, R. (2007) Distinct structural and functional roles of conserved residues in the first extracellular domain of receptors for corticotropin releasing factor and related G-protein coupled receptors. J.Biol.Chem., 282:37529-37536.

Grace CR, Perrin MH, Cantle JP, Vale WW, Rivier JE, Riek R. (2007) Common and divergent structural features of a series of corticotropin releasing factor-related peptides. J.Am.Chem.Soc. 129:16102-14.

Allendorph, G.P., Isaacs, M.J., Kawakami, Y., Belmonte, J.C., Choe, S. (2007) BMP-3 and BMP-6 Structures Illuminate the Nature of Binding Specificiy with Receptors. Biochemistry,46, 12238-12247.

Grace, C.R., Cervini, L., Gulyas, J., Rivier, J., Riek, R. (2007) Astressin-amide and astressin-acid are structurally different in dimethylsulfoxide. Biopolymers, 87, 196-205.

Blain, K.Y., Kwiatkowski, W., Zhao, Q., Fleur, D.L., Naik, C., Chun, T.W., Tsareva, T., Kanakaraj, P., Laird, M.W., Shah, R., George, L., Sanyal, I., Moore, P.A., Demeler, B., Choe, S. (2007) Structural and Functional Characterization of CC Chemokine CCL14. Biochemistry, 46, 10008-10015.

Grace, C.R., Perrin, M.H., Gulyas, J., Digruccio, M.R., Cantle, J.P., Rivier, J.E., Vale, W.W., Riek, R. (2007) Structure of the N-terminal domain of a type B1 G protein-coupled receptor in complex with a peptide ligand. Proc Natl Acad Sci U S A. 2007 104, 4858-63.

George Allendorph, Wylie Vale, Senyon Choe (2006) Structure of the ternary signaling complex of a TGF-beta superfamily member. Proc. Natl. Acad. Sci. 103, 7643-8.

Brown, M. A., Zhao, Q., Baker, K.A., Naik, C., Chen, C., Pukac, L., Singh, M., Tsareva, T., Parice, Y., Mahoney, A., Roschke, V., Sanyal, I., Choe, S. (2005) Crystal structure of BMP-9 and functional interactions with pro-region and receptors.. J. Biol. Chem., 280, 25111-8.

Grace, C.R., Perrin, M.H., DiGruccio, M.R., Miller, C.L., Rivier, J.E., Vale, W.W., Riek, R. (2004) NMR structure and peptide hormone binding site of the first extracellular domain of a type B1 G protein-coupled receptor. Proc Natl Acad Sci U S A. 2004 101,12836-41.

Greenwald J., Vega M.E., Allendorph G.P.,Fischer W.H., Vale W., Choe S. (2004) A flexible activin explains the membrane-dependent cooperative assembly of TGF-B family receptors. Molecular Cell, 15, 485-9.

Groppe J., Greenwald J., Wiater E., Rodriguez-Leon J., Economides AN., Kwiatkowski W., Baban K., Affolter M., Vale WW., Belmonte JC., Choe S. (2003) Structural basis of BMP signaling inhibition by Noggin, a novel twelve-membered cystine knot protein J.Bone & Joint Surgery 85, 52-58.

Greenwald, J.,  Groppe, J., Gray, P.,Wiater, E., Kwiatkowski, W., Vale, W., Choe, S. (2003) The BMP//ActRII extracellular domain complex provides new insights into the cooperative nature of receptor assembly. Molecular Cell, 11, 605-617.

Groppe,J., Greenwald, J.,  Wiater, E., Rodriguez-Leon, J.,Economides, A., Kwiatkowski, W., Affolter, M., Vale, W., I.-Belmonte, J.-C., Choe, S. (2002) Structural basis of BMP signaling inhibition by Noggin, a novel cystine knot protein. Nature, 420,636-642.

Blanchoin, L., Robinson, R., Choe, S., Pollard, T. (2000) Phosphorylation of Acanth-amoeba actophorin (ADF/cofilin) blocks interaction with actin without structural change. J. Mol. Biol., 295, 203-211.

Wesche, J., Iedlocha, A., Falnes, P.O., Choe, S., Olsnes, S. (2000) Externally Added aFGF mutants do not require extensive unfolding for transport into the cytosol and the nucleus of NIH/3T3 cells. Biochemistry, 39, 15091-15100.

Robinson, R.C., Radziejewski, C., Spraggon, G., Greenwald, J., Kostura, M., Burtnick, L.D., Stuart, D.I., Choe, S., Jones, Y. (1999) The structures of the brain-derived neutrotrophic factor/neurotrophin 4 (NT4) heterodimer and the NT3 and NT4 homodimers reveal a common Trk-binding site. Protein Sci. , 8, 2589-2597.

Greenwald, J., Fischer, W., Vale, W.W., Choe, S. (1999) Three finger toxin fold for the extracellular ligand-binding domain of type II activin receptor serine kinase. Nature Struc. Biol., 6, 18-22.

Fischer, W., Greenwald, J., Park, M., Craig, A.G., Choe, S., Vale, W. (1999) The disulfide arrangement in the extracellular domain of the activin type II receptor. J. Protein Chemistry, 18, 437-46.

Robinson, R., Mejilano, M., Le, V., Burtnick, L.D., Yin, H.L., Choe, S. (1999) Domain movement in gelsolin: a Ca-activated switch. Science, 286, 1939-1942.

Ryan, A., Blumberg, B., Rodriguez-Estaban, C., Yonie-Tamura, S., Tamura, K., Tsukui, T., Peña, J., Sabbagh, W., Greenwald, J., Choe, S., Norris, D.P., Robertson, E.J., Evans, R.M., Rosenfeld, M.G., Belmonte, J. (1998) Pitx2 determines left-right asymmetry of internal organs in vertebrates. Nature 394, 545-51.

Greenwald, J., Le, V., Corrigan, A., Fischer, W., Komives, E., Vale, W., Choe, S. (1998) Characterization of the extracellular ligand-binding domain of the type II activin receptor serine kinase. Biochemistry, 37, 16711-8.