Determination of (h2)J(NN) and (h1)J(HN) coupling constants across Watson-Crick base pairs in the Antennapedia homeodomain-DNA complex using TROSY -- Web of Science version 4.1 (ISI2)

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Determination of (h2)J(NN) and (h1)J(HN) coupling constants across Watson-Crick base pairs in the Antennapedia homeodomain-DNA complex using TROSY
Pervushin K, Fernandez C, Riek R, Ono A, Kainosho M, Wuthrich K
JOURNAL OF BIOMOLECULAR NMR 16: (1) 39-46 JAN 2000

Document type: Article

Language: English

Cited References: 29

Times Cited: 0

Abstract:
This paper describes NMR measurements of N-15-N-15 and H-1-N-15 scalar couplings across hydrogen bonds in Watson-Crick base pairs, (h2)J(NN) and (h1)J(HN), in a 17 kDa Antennapedia homeodomain-DNA complex. A new NMR experiment is introduced which relies on zero-quantum coherence-based transverse relaxation-optimized spectroscopy (ZQ-TROSY) and enables measurements of (h1)J(HN) couplings in larger molecules. The (h2)J(NN) and (h1)J(HN) couplings open a new avenue for comparative studies of DNA duplexes and other forms of nucleic acids free in solution and in complexes with proteins, drugs or possibly other classes of compounds.

Author Keywords:
hydrogen bonds, nucleic acid structure, scalar coupling, TROSY

KeyWords Plus:
RELAXATION-OPTIMIZED SPECTROSCOPY, NMR-SPECTROSCOPY, CHEMICAL-SHIFT, PYROCOCCUS-FURIOSUS, HYDROGEN-BONDS, SENSITIVITY, RUBREDOXIN, TENSORS, BINDING, PULSES

Addresses:
Wuthrich K, ETH Honggerberg, Inst Mol Biol & Biophys, CH-8093 Zurich, Switzerland.
ETH Honggerberg, Inst Mol Biol & Biophys, CH-8093 Zurich, Switzerland.
Tokyo Metropolitan Univ, Fac Sci, Dept Chem, Tokyo 19203, Japan.

Publisher:
KLUWER ACADEMIC PUBL, DORDRECHT

IDS Number:
279AK

ISSN:
0925-2738

Article 2 of 27